Application of a high-throughput quantitative PCR system for simultaneous monitoring of SARS-CoV-2 variants and other pathogenic viruses in wastewaterArticle Published on 2022-12-202022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] acute respiratory syndrome Alpha variant analyzed application applied assays can be used collected Community coronavirus coronavirus disease COVID-19 delta variant demonstrated detect eight epidemiologically FIVE Health High-throughput qPCR highlighting increase Japan L452R labor-intensive likelihood Mie N501Y nine nucleotide nucleotide substitution pathogenic virus pathogenic viruses Pathogenic viruses, SARS-CoV-2 Patient PCR positive prevalent qPCR quantitative PCR quarantine reported RNA RT-qPCR SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant separated Surveillance target tested time-consuming Treatment virus viruses Volume wastewater Wastewater. Yokkaichi [DOI] 10.1016/j.scitotenv.2022.158659 PMC 바로가기
Peptide microarrays coupled to machine learning reveal individual epitopes from human antibody responses with neutralizing capabilities against SARS-CoV-2기계 학습과 결합된 펩티드 마이크로어레이는 SARS-CoV-2에 대한 중화 기능을 가진 인간 항체 반응의 개별 에피토프를 나타냅니다.Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료법, [키워드] anti-SARS-CoV-2 antibody Antibody binding assays binding epitope capture causative agent cellular coronavirus coronavirus SARS-CoV-2 Course COVID-19 detectable epitope Epitopes human antibody response IgA IgG IgM antibody immunoassays incubated infected with SARS-CoV-2 machine learning Microarray N-terminus negative control samples neutralization neutralization assays Neutralizing Neutralizing antibodies nine overlapping Patient peptide peptide arrays peptides positive protective effect Protective effects random Random forest response S protein SARS CoV SARS CoV-2 SARS-CoV-2 sera serology Serology. Specific structural protein structural proteins the disease the S protein vaccine candidate vaccine candidates Viral [DOI] 10.1080/22221751.2022.2057874 PMC 바로가기 [Article Type] Article
Highly sensitive and rapid detection of SARS-CoV-2 via a portable CRISPR-Cas13a-based lateral flow assayArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome addition amplification approach assays clinical sample comparable component Concentration coronavirus disease Coronavirus-2 COVID-19 CRISPR Diagnostic method Effectiveness ENhance examined identify immunochromatographic IMPROVE individual isothermal lateral flow lateral flow assay lateral flow strip limit of detection molecular polymerase chain primer probe Protein provide Quality control reliability Result reverse transcription RNA RT-PCR SARS-CoV-2 screened sensitive detection. sensitivity significantly Spread tested virus detection [DOI] 10.1002/jmv.28096 PMC 바로가기
Preclinical efficacy and safety of novel SNAT against SARS-CoV-2 using a hamster modelArticle Published on 2022-12-012022-11-15 Journal: Drug delivery and translational research [Category] COVID19(2023년), SARS, 치료제, [키워드] acute respiratory syndrome antioxidant Antiviral antiviral drug Antiviral drug candidate assays Body weight loss coronavirus COVID-19 demonstrated Efficacy Efficacy and safety Epidemic fibroblast global public health hamsters Health Histopathology human cells human lung epithelium IMPROVE Inhaled inhaler LDH lipid peroxidation lung MDA Nanotechnology oral swabs other respiratory virus pandemic platform Preclinical reduce SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-infected hamsters silver nanoparticle SmaRT SNAT. tested virus load virus titer [DOI] 10.1007/s13346-022-01166-x PMC 바로가기
Zinc pyrithione is a potent inhibitor of PL Pro and cathepsin L enzymes with ex vivo inhibition of SARS-CoV-2 entry and replication아연 피리티온은 SARS-CoV-2 진입 및 복제를 생체 외에서 억제하는 PL Pro 및 카텝신 L 효소의 강력한 억제제입니다.Article Published on 2022-12-012022-09-11 Journal: Journal of Enzyme Inhibition and Medicinal Chemist [Category] COVID19(2023년), SARS, 치료제, [키워드] analogue Anti-SARS-CoV-2 Activity Antiviral antiviral activity Antiviral agents assays cathepsin L comparable complex complexes contrary COVID-19 effective enzyme Enzymes evaluated Ex vivo expressed human lung tissue in vitro inhibition inhibitor involved Ligand media Pro pyrithione Replication ruthenium SARS-CoV-2 SARS-CoV-2 entry stability synthesised therapeutic therapeutic agent Zinc Zinc complexes Zinc pyrithione zinc. [DOI] 10.1080/14756366.2022.2108417 PMC 바로가기 [Article Type] Article
Validation of SARS-CoV-2 pooled testing for surveillance using the Panther Fusion® system: Impact of pool size, automation, and assay chemistryArticle Published on 2022-11-072022-11-15 Journal: PLoS ONE [Category] SARS, 진단, 치료기술, [키워드] approach assays authorization automated conducted cut-off threshold cycle threshold decrease demonstrated diagnostic Emergency evaluated expected FDA FIVE generate Impact less limit of detection lowering nasopharyngeal sample nucleic acid amplification positive positive sample resources SARS-CoV-2 SARS-CoV-2 RNA sensitivity specificity specimen Surveillance surveillance testing System tested validation was used [DOI] 10.1371/journal.pone.0276729 PMC 바로가기
The evaluation of an automated mariPOC SARS-CoV-2 antigen test compared to RT-qPCR SARS-CoV-2 assay and comparison of its sensitivity in Delta- and Omicron-variant samplesArticle Published on 2022-11-012022-11-16 Journal: Influenza and Other Respiratory Viruses [Category] SARS, 변종, [키워드] 95% CI 95% confidence interval Affect analysed Antigen antigen testing assays Asymptomatic automated circulating clinical samples cycle threshold Delta diagnostics evaluate evaluated genomic investigated lineages material mild respiratory symptom Mutation Nasopharyngeal swab omicron oropharyngeal swabs performed positive retrospective RT-qPCR SARS-CoV-2 SARS-CoV-2 antigen sensitivities sensitivity Sensitivity and specificity Sensitivity. specificity subject the SARS-CoV-2 variant variants [DOI] 10.1111/irv.13048 PMC 바로가기
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccinationArticle Published on 2022-11-012022-11-15 Journal: Journal of Immunological Methods [Category] SARS, 진단, 치료기술, 치료제, [키워드] Antibody Response Antigen antigenic target assays correlated COVID-19 IgG immune response increasingly Infection infection with SARS-CoV-2 measure measurement multiplex Multiplex assay nucleocapsid antigen performance platform Quantitative ranged RBD Receptor binding domain S1 and S2 SARS-CoV-2 SARS-COV-2 infection sensitivity serologic assay Serologic response Serology. specificity subunit vaccination Variation [DOI] 10.1016/j.jim.2022.113345 PMC 바로가기
Immunogenicity and reactogenicity of SARS-CoV-2 vaccines in people living with HIV in the Netherlands: A nationwide prospective cohort studyArticle Published on 2022-10-272022-11-15 Journal: PLoS Medicine [Category] COVID19(2023년), SARS, 진단, [키워드] 95% CI Ad26.COV2.S Ad26.COV2.S vaccine age Analysis anti-SARS-CoV-2 Anti-spike antibody Antibody Response Antiretroviral therapy approved assays BNT162b2 CD4+ T-cell CD8+ T-cell ChAdOx1-S comparable Concentration conducted Control controls COVID-19 defined ELISPOT Endpoint Environment Final generally mild geometric HIV HIV-negative IFN-γ immunogenic immunogenicity Increased IQR less maintain male marker median age mRNA vaccination mRNA vaccine mRNA-1273 mRNA-based vaccines Netherland Netherlands of BNT162b2 PLWH predicted primary endpoint prospective cohort study Public reactogenicity receiving recipients reduced Registered remained required response SARS-CoV-2 antibody SARS-CoV-2 IgG SARS-CoV-2 spike peptide SARS-CoV-2 vaccination SARS-CoV-2 vaccine secondary Serious Adverse Events serological response Sex suppressed T-cell Response the median the vaccine Trial vaccination Vaccine vaccine group Viral load with HIV [DOI] 10.1371/journal.pmed.1003979 PMC 바로가기
Concordance of SARS-CoV-2 Antibody Results during a Period of Low PrevalenceArticle Published on 2022-10-262022-11-15 Journal: mSphere [Category] COVID19(2023년), SARS, 진단, [키워드] 95% confidence interval Abbott acute respiratory syndrome Analysis anti-SARS-CoV-2 anti-SARS-CoV-2 antibody anti-SARS-CoV-2 IgG Antigen antigens Architect assays CDC collected combinations Concentration Concordance contributed coronavirus coronavirus disease COVID-19 Elecsy ELISA enzyme-linked immunosorbent evaluate False positivity first test Frequency IgG IgG antibodies immunoassay immunoassays Immunoglobulin individual investigated low prevalence nucleocapsid nucleocapsid protein pandemic participant Participants performed Period positive positive result Prevalence produced proportion quantified Research Result Roche SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 IgG SARS-CoV-2 IgG antibody sequential test sera serological test seropositive specimen Spike protein spike protein. subset Support target tested the SARS-CoV-2 The United States Vaccine vaccine availability [DOI] 10.1128/msphere.00257-22 PMC 바로가기